Urinary Aspartate Transaminase in Childhood

Urinary aspartate transaminase in childhood.

Dffluoro-oxaloacetate with Aspartate Transaminase

Difluoro-oxaloacetate interacts with the aldimine form of aspartate transaminase to give a complex, the dissociation constant ofwhich has been determined spectrophotometrically and by 19F n.m.r. (nuclear magnetic resonance). The 19F n.m.r. line-width-pH and chemical-shift-pH profiles of difluoro-oxaloacetate in the presence of the aldimine form of the enzyme both show inflexion points in the pH...

Urinary Aspartate Transaminase (ast) Activity and Detection of Its Inhibitors in Normal Human Urine

An overall 176% increase in enzyme activity followed byS ephadex G-25 (fine) whole urine fractionation and a highly significant (P L,001) partial inhibition by earlier Sephadex fractions and maximum inhibition by earlier Sephadex fractions and maximum inhibition of enzyme by fraction 7 have suggested the presence of both high and low molecular weight urinary inhibitors of aspartate-transaminase...

Serum transaminase variations in childhood.

The study of serum transaminases has recently entered clinical practice. In December, 1954, Wroblewsky and La Due demonstrated an increase of glutamic-oxalacetic (GOT) transaminase in the serum of patients suffering from various liver diseases and in carbon tetrachloride intoxication. In 1955, de Ritis, Coltorti and Giusti demonstrated an increase of both aspartic-ketoglutaric (GOT) and alanine...

Differential scanning calorimetry of cytoplasmic aspartate transaminase.

Differential scanning calorimetry has been applied to study factors affecting the thermally induced denaturation of cytoplasmic aspartate aminotransferase, a dimeric pyridoxal enzyme. The consequences of binding of coenzyme and substrate derivatives to both the apo and holo forms of the enzyme were investigated and are interpreted in terms of the stabilization of the native form of the enzyme. ...